• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 22 Issue 1
Apr.  2012
Turn off MathJax
Article Contents
LIN Zhi-min, Song Ya-na, YAO Mei-bin, CHEN Bin, ZHENG Wei-wen. PCR detection and characterization of a phenol-degrading bacteria(Alcaligenes faecalis BC2001)[J]. Fujian Journal of Agricultural Sciences, 2007, 22(1): 50-53.
Citation: LIN Zhi-min, Song Ya-na, YAO Mei-bin, CHEN Bin, ZHENG Wei-wen. PCR detection and characterization of a phenol-degrading bacteria(Alcaligenes faecalis BC2001)[J]. Fujian Journal of Agricultural Sciences, 2007, 22(1): 50-53.

PCR detection and characterization of a phenol-degrading bacteria(Alcaligenes faecalis BC2001)

  • Received Date: 2006-11-03
  • Rev Recd Date: 2006-12-11
  • Publish Date: 2007-03-15
  • A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.A 684 bp fragment was amplified from Alcaligenes faecalis BC2001’s total DNA.After sequencing and alignment by Blast,the sequence share 97% homology with phenol-degrading Proteobacterium L-18 strain(accession number:AY346142).Another phenol-degrading experiment showed that pH 6 was optimum for the growth of BC2001.It exemplified the BC2001 strain showing its phenol-degrading ability at 300 mg·L-1 of phenol concentration.
  • loading
  • [1]
    KLIBANOV A M,TU T M.SCOTT K P.Peroxidase-cacalyzed removahl phenols from calconversion wastewaters[J].Science,1983,221:259-261.
    [2]
    HOPPER D J,CHAPMAN P J,DAGLEY S.Metabolismof L-malate and D-malate by a species of Pseudomonas[J].Bacteriol,1970,104,1197-1202.
    [3]
    ARAI H,AKAHIRA S,OHISHI T,et al.Adaptation of Comamonas testosteroni TA441 to utilize phenol:organization and regulation of the genes involved in phenol degradation[J].Microbiology,1998,144,2895-2903.
    [4]
    WATANABE K,HINO S,ONODERA K,et al.Diversity in kinetics of bacterial phenol-oxygenating activity[J].Ferment.Bioeng,1996,81,560-563.
    [5]
    BASTOS A E,MOON D H,ROSSI A,et al.Salt tolerant phenol degrading microorganisms isolated from Amazonian soil samples[J].Arch MicrobioL,2000,174(5):346-352.
    [6]
    STATE ENVIRONMENTAL PROTECTION ADMINISTRATION OF CHINA.The monitoring and analysis methods of water and waste water[J].Beijing:China Environment Science Press,1997,408-410.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2482) PDF downloads(717) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return