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Volume 26 Issue 4
Nov.  2011
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Article Contents
WANG Ding-feng, ZENG Ming-sen, WANG Qing-sen, LIU Feng-jing, WU Guang-yuan. Cloning and Sequence Analysis on A Chitinase Gene of Beauveria bassiana Isolated from Homona coffearia[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 507-512.
Citation: WANG Ding-feng, ZENG Ming-sen, WANG Qing-sen, LIU Feng-jing, WU Guang-yuan. Cloning and Sequence Analysis on A Chitinase Gene of Beauveria bassiana Isolated from Homona coffearia[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 507-512.

Cloning and Sequence Analysis on A Chitinase Gene of Beauveria bassiana Isolated from Homona coffearia

  • Received Date: 2011-04-13
  • Rev Recd Date: 2011-05-18
  • Publish Date: 2011-08-15
  • Beauveria bassiana is one of the most important entomopathogenic fungi.It plays a crucial role in biocontrol of the farming and forestry pests.The chitinase gene,Bbchit1,was cloned from JYBb201-11 isolate of Beauveria bassiana,which is highly virulent to Homona coffearia.The expected DNA fragments were amplified by DNA-PCR,and the total RNA-RT-PCR with a pair of primers.The fragments were introduced into DH5 isolate of Escherichia coli by PMD18-T vector,and sequenced subsequently.The results from DNA-PCR and the total RNA-RT-PCR showed that the fragments were both 1047 bp.The gene consisted of an open reading frame with 1047 bp(GenBank accession NO.HQ435871),encoding 348 amino acids,which contains an N-terminal 22 amino acid residue displaying the characteristics of a signal peptide.The mature chitinase had a molecular mass of 36.78 kD and a calculated pI of 5.95.The protein sequence contained two conserved regions including a putative substrate binding site(SIGG)and catalytic domain(DGIDIDIE)of fungal chitinases.The chitinase belonged to the class V of family 18 of glycosyl hydrolases.The analysis showed that the deduced amino acid sequence was 99.43% homologous to that of B.bassiana strain Bb0062(AAN41259)and B.bassiana strain NCIM1216(ACF32998),and 98.28% to that of B.bassiana strain MTCC 2028(ACZ28129).
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    王定锋,吴光远,王庆森,等. 茶卷叶蛾寄生真菌的分离、鉴定及毒力测定[J]. 福建农业学报, 2010, 25(3):344-349.
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    汤强,章玉萍,谢翎,等. 玫烟色棒束孢几丁质酶的转基因球孢白僵菌菌株的获得及其对马尾松毛虫的毒力增效作用[J]. 昆虫学报, 2009, 52(7):755-762.
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