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Volume 22 Issue 1
Apr.  2012
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Article Contents
MENG Fan-lei, DIAO You-xiang, WANG Hui, WANG Ni, LIU Fang-na, LIU Xia. Establishment and application of digoxigenin-labeled nucleic acid probe for detection of infectious bronchitis virus[J]. Fujian Journal of Agricultural Sciences, 2007, 22(1): 39-42.
Citation: MENG Fan-lei, DIAO You-xiang, WANG Hui, WANG Ni, LIU Fang-na, LIU Xia. Establishment and application of digoxigenin-labeled nucleic acid probe for detection of infectious bronchitis virus[J]. Fujian Journal of Agricultural Sciences, 2007, 22(1): 39-42.

Establishment and application of digoxigenin-labeled nucleic acid probe for detection of infectious bronchitis virus

  • Received Date: 2006-11-30
  • Rev Recd Date: 2007-01-09
  • Publish Date: 2007-03-15
  • According to the genomic sequences of N gene of infectious bronchitis virus(IBV) published in Genbank,a pair of primers was designed for amplifying the 582 bp fragment in RT-PCR experiments.The PCR product was labeled with digoxigenin as DNA probe for detection of IBV.The hybridization assay of specificity showed all RNA of IBV strains were positive,but other nucleotide extracted from NDV and PMV negative.The sensitivity test showed that as low as 10 pg of IBV’s RNA could be detected by DIG-labeled probe.Therefore,it was concluded that the DIG-labeled probe could be used for IBV detection.
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  • [1]
    LEE C W,JACKWOOD M W.Origin and evolution of Georgia 98(GA98),a new serotype of avian infectious bronchitis virus[J].Virus Res,2001,80(1-2):33-39.
    [2]
    孟凡磊,刁有祥,王辉,等.鸡传染性支气管炎变异株(793/B)核蛋白基因的克隆与序列分析[J].中国病毒学,2006,21(5):468-471.
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