• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 22 Issue 2
Apr.  2012
Turn off MathJax
Article Contents
LIU Fu-ping, CHEN Yi-liang. Effect of cytokinin on the induction and macromolecules metabolic kinetics of somatic embryogenesis in Phalaenopsis sp.[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 162-166.
Citation: LIU Fu-ping, CHEN Yi-liang. Effect of cytokinin on the induction and macromolecules metabolic kinetics of somatic embryogenesis in Phalaenopsis sp.[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 162-166.

Effect of cytokinin on the induction and macromolecules metabolic kinetics of somatic embryogenesis in Phalaenopsis sp.

  • Received Date: 2007-02-13
  • Rev Recd Date: 2007-03-28
  • Publish Date: 2007-06-15
  • Two ex-plants from test tube seedling of Phalaenopsis sp.were cultured on the media containing 6-BA and 2,4-D.From the stem ex-plant,embryogenic callus(EC)with large amounts of starch granules in cell could be induced with the presence of 6-BA.On the other hand,the callus induced with 2,4-D or with both 2,4-D and 6-BA were unable to differentiate.EC could also be induced efficiently from the leaf ex-plant cultured on the medium containing 6-BA.Histological observation and metabolic analysis conducted during somatic embryogenesis showed that,in leaf ex-plant,the DNA content increased slowly initially and sharply on the 25th day of induction.The RNA content increased when induction began,and reached a peak on the 11-18th day.The soluble protein content started to change later than RNA and peaked on the 25th day.
  • loading
  • [1]
    谷祝平,严廷进.大花蕙兰茎尖组织培养及其形态建成的研究[J].实验生物学报,1989,22(2):149-155.
    [2]
    崔凯荣,戴若兰.植物体细胞胚发生的分子生物学[M].北京:科学出版社,2000.
    [3]
    SAIPRASAD G V S.et al.Propagation of three orchid genera using encapsulationted protocorm-like bodies[J].In vitro Cell Dev,Biol,-Plant,2003,39:42-48.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2132) PDF downloads(592) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return