Genetic Diversity and Principal Components of Tea Germplasms from Shiting Plantations in Nan'an

OU Xiaoxi; LIN Hongzheng; HE Jihang; LI Qiuming; FU Ruidian; CHEN Yucai; ZHENG Yucheng; SUN Yun

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OU X X, LIN H Z, HE J H, et al. Genetic Diversity and Principal Components of Tea Germplasms from Shiting Plantations in Nan'an [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−10

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OU X X, LIN H Z, HE J H, et al. Genetic Diversity and Principal Components of Tea Germplasms from Shiting Plantations in Nan'an [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−10

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Genetic Diversity and Principal Components of Tea Germplasms from Shiting Plantations in Nan'an

OU Xiaoxi^1
,,
LIN Hongzheng¹,
HE Jihang¹,
LI Qiuming¹,
FU Ruidian²,
CHEN Yucai³,
ZHENG Yucheng^{1, 4
,
,},
SUN Yun^{1
,
,}

1.
College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Fujian Province, Fuzhou, Fujian　350002, China
2.
Nanan Shiting Green Tea Research Association, Quanzhou, Fujian　361000, China
3.
Nanan Fengzhou Town Agricultural Service Center, Quanzhou, Fujian　362000, China
4.
School of Tea and Food Science, Wuyi University,/Tea Science Research Institute, Wuyi University, Wuyishan, Fujian　354300, China

Received Date: 2024-07-15
Accepted Date: 2024-09-02
Rev Recd Date: 2024-09-01

Available Online: 2024-11-11

Abstract

Abstract

Objective Genetic diversity of the tea germplasms preserved at Shiting plantations in Nan’an was studied for promoting the utilization of the local specialty. Methods SNP molecular marker technology was applied to analyze the genetic diversity and relationships of 17 tea germplasms from the Shiting plantations and 6 representative varieties from Fujian. HPLC and UPLC-MS/MS were employed to determine the contents of catechins and free amino acids in 10 selected samples for a comparison among the tea varieties. Results Forty-four SNP loci suitable for identifying the tea plants were selected. An SNP fingerprint profile of the germplasms was constructed. The UPGMA evolutionary tree divided the 23 germplasms into 4 subgroups, which showed a close relation with Rougui and Qidan teas from northern Fujian. Significant differences were found on the contents of major amino acids and catechins among the varieties (P＜0.05) with total free amino acids from 8.46 mg·g⁻¹ to 32.66 mg·g⁻¹ and catechins ranging from 107.56 mg·g⁻¹ to 177.60 mg·g⁻¹. The germplasms coded ST2, ST3, ST6, and ST17 had high contents of EGCG or EGCG3"Me, while ST3 the most on amino acids and the least on catechin bitterness index. Conclusion Shiting plantations in Nan’an had a rich collection of tea germplasms that were closely related to those in northern Fujian. Among them, ST2, ST3, ST6, and ST17 teas had high contents of either EGCG or EGCG3"Me. ST3, specifically, appeared to be suitable for making green tea as well as in breeding programs.
- Shiting, Nan’an,
- Camellia sinensis,
- germplasm,
- genetic diversity,
- component analysis,
- SNP

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References(34)

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