The use of RAPD markers in population genetic analyses of cage-cultured Pseudosciaena crocea(Richardson) from Fujian,China

Genetic variation among three samples of cage-cultured,Pseudosciaena crocea from Luoyuan and Pingtan,Fujian were investigated using RPAD techniques.Four OPV random primers were employed for DNA amplification through PCR.There were 39 loci detected and their representative bands were labeled as genetic markers for population genetic analyses.Forteen loci were found to be monomorphic.They were considered potential candidates for the selection of species-specific genetic markers.On average,each sample had 48.2% polymorphic loci.Nei’s average heterozygosities (H) for respective samples were 0.2515,0.2755,and 0.2575.Fishes from Pingtan and Luoyuan collected in 2004 displayed conspicuous differences in genotype frequency at many of the observed loci,indicating that the population of caged fish could differentiate in genetic structures to a certain extent.Meanwhile,the present PCR results worked out with OPV-02 revealing two distinguishable electrophoregrams that separated samples from Luoyuan collected in 2004 and in 2005.It is proposed herein that this OPV-02 distinction might be the first evidence that demonstrates genetic divergence between the so-called geographic races of Pseudosciaena crocea along the eastern coast of China.