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Volume 22 Issue 2
Apr.  2012
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Article Contents
WEN Lei, SHI Hong, XU Hong, ZHANG Jun. High-density Cultures and Identification of the Transgenic Spirulina platensis[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 126-129.
Citation: WEN Lei, SHI Hong, XU Hong, ZHANG Jun. High-density Cultures and Identification of the Transgenic Spirulina platensis[J]. Fujian Journal of Agricultural Sciences, 2007, 22(2): 126-129.

High-density Cultures and Identification of the Transgenic Spirulina platensis

  • Received Date: 2006-12-20
  • Rev Recd Date: 2007-01-26
  • Publish Date: 2007-06-15
  • The transgenic Spirulina platensis constructed in our lab was cultured in a PhR-L20C photobioreactor designed by the Institute of Process Engineering,Chinese Academy of Science.The conditions were optimized and applied for the high-density cultivation within the parameters of this photobioreactor.The initial pH was about 9.0,light intensity 10000 Lx,and the temperature 28-30℃.The result showed that the average optical density(OD560) reached 3.0 after 10 days with a growth rate of 0.3 OD reading per day.The final average dry weight yield of S.platensis was 1.3 g·L-1.After cultivation for 30 days without the aid of the antibiotic,G418,in the photobioreactor,the target gene was positively detected by means of PCR on the genome of S.platensis.In addition,the Western-blot result proved the foreign gene was effectively expressed in the transgenic S.platensis.
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    LOU S L,LIU G F,ZHANG J.Genetics and molecular biology of algae Amsterdam[M].Netherland:SPB Academic Publishing,1996:421.
    [6] 周克夫,章军,陈天圣,等.胸腺素α1抗体的制备和鉴定[J].中国免疫学杂志,2001,17(1):43-47.
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