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ZHAI Huan-chen, SHI Huai, SONG Ya-na, ZHENG Wei-wen. Simultaneous determination of seven organic acids in Prunus mume with reverse-phase high performance liquid chromatography[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 414-417.
Citation:
ZHAI Huan-chen, SHI Huai, SONG Ya-na, ZHENG Wei-wen. Simultaneous determination of seven organic acids in Prunus mume with reverse-phase high performance liquid chromatography[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 414-417.
ZHAI Huan-chen, SHI Huai, SONG Ya-na, ZHENG Wei-wen. Simultaneous determination of seven organic acids in Prunus mume with reverse-phase high performance liquid chromatography[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 414-417.
Citation:
ZHAI Huan-chen, SHI Huai, SONG Ya-na, ZHENG Wei-wen. Simultaneous determination of seven organic acids in Prunus mume with reverse-phase high performance liquid chromatography[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 414-417.
A method for simultaneous and rapid determination of 7 organic acids(i.e.,oxalic,tartaric,malic,lactic,acetic,citric and succinic acid)in Prunus mume with reverse-phase high performance liquid chromatography(HPLC)was developed.HPLC was performed on a Hypersil ODS C18 column(250mm×4.6mm i.d.,5 μm)at 21℃,using KH2PO4(0.01 mol·L-1;pH 2.8)as mobile phase at the flow rate of 0.8 mL·min-1 and the detection wavelength of 215 nm.The recoveries of the organic acids ranged from 75.4% to 121.0% with relative standard deviations of 0.78%-2.12% and the limits of detection 0.022-1.290 μg·mL-1.The results of the 7 organic acids in 4 P. mume samples are presented.
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