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Volume 22 Issue 4
Mar.  2012
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SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.
Citation: SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.

PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria

  • Received Date: 2007-07-26
  • Rev Recd Date: 2007-09-04
  • Publish Date: 2007-12-15
  • Eight bacterial isolates,which originated from paddy fields in Fujian,were analyzed using 16SrDNA-PCR-DGGE.Through sequencing and Blast analysis,9 N2-fixing bacteria strains were believed to belong to Mesorhizobium sp.,Sphingomonas sp.,Rhizobium sp.,Bradyrhizobium sp.,Klebsiella sp.,Burkholderia cepacia,Pseudomonas sp.,Nocardioides and Rhodococcus sp.,due to their high homology with the related accessions in Genbank.
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    WATANABE K,KIDAMA Y,HARAYAMA S.Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting[J].Microbiol Methods,2001,44(3):253-262.
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