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Volume 22 Issue 4
Mar.  2012
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ZHAO Jian, GAO Xiao-dan, QIU Rong-zhou, WENG Qi-yong. Optimization of medium formulation and conditions for growing biocontrol bacteria ZB-6 using Uniform Design[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 341-345.
Citation: ZHAO Jian, GAO Xiao-dan, QIU Rong-zhou, WENG Qi-yong. Optimization of medium formulation and conditions for growing biocontrol bacteria ZB-6 using Uniform Design[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 341-345.

Optimization of medium formulation and conditions for growing biocontrol bacteria ZB-6 using Uniform Design

  • Received Date: 2007-07-26
  • Rev Recd Date: 2007-09-12
  • Publish Date: 2007-12-15
  • ZB-6,a Bacillus subtilis strain obtained from healthy tomato leaf,showed a strong inhibitory activity toward plant pathogens.The Uniform Design was used to optimize the medium formulation for ZB-6 fermentation in shaking flasks.The identified formulation was∶sugar 4.82 g,beef extract 3.15 g,bean cake powder 11.01 g,ammonium nitrate 5.21 g,dipotassium hydrogen phosphate 5.04 g,and potassium dihydrogen phosphate 2.84 g.It increased the ZB-6 biomass concentration from 2.36×108 to 6.7×109 cfu·mL-1.
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    刘莉,辛振祥.配方优化设计方法简介[J].橡塑技术与装备,2004,30(10):8-12.
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