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ZHU Xiao-lin, WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, CHEN Shi-long, ZHU Xiao-li, LI Zhao-long. Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 925-929.
Citation:
ZHU Xiao-lin, WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, CHEN Shi-long, ZHU Xiao-li, LI Zhao-long. Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 925-929.
ZHU Xiao-lin, WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, CHEN Shi-long, ZHU Xiao-li, LI Zhao-long. Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 925-929.
Citation:
ZHU Xiao-lin, WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, CHEN Shi-long, ZHU Xiao-li, LI Zhao-long. Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 925-929.
In this study, we report the cloning and sequencing of M gene of porcine transmissible gastroenteritis virus Fujian strain (TGEV-FJ), and analyze the homology, signal peptide, glycosylation sites, phosphorylation sites, secondary structure of M protein by using bioinformatics software. The complete M gene in TGEV-FJ strain was successfully amplified which has 789 bp, encoding 262 amino acids and then constructed into the recombinant plasmid pGEM-T-M. Sequential analysis and phylogenetic studies demonstrated that the TGEV M gene has highly conservative and consanguineous between TGEV-FJ strain and HN2002 strain. The prediction results showed that M protein in TGEV-FJ strain had a signal peptide cleavage site (ACG16-17ER), and 3 potential N-glycosylation sites,6 serine phosphorylation sites,1 threonine phosphorylation sites and 5 tyrosine phosphorylation sites. The secondary structure of M protein prediction showed that alpha helix, beta sheet and random coil were 11.59%, 44.93% and 43.48%, respectively. The results of the present study would lay some basic molecular biological foundation for prokaryotic expression of M gene in TGEV-FJ strain, which would subsequently facilitate further research for molecular diagnosis and gene engineering vaccines development.
ESCORS D,ORTEGO J,ENJUANES L,et al.The membrane M protein car boxy terminal binds to transmissible gastroenteritis coronavirus core and contributes to core stability[J].J Virol,2001,75(3):1312-1324.