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CHEN Hui, CHEN Mei-xia, CHEN Yan-ping, WEI Cheng-lin, LIAO Ying-min, CHEN Fu-cheng, TAO Ai-fen, XU Jian-tang, QI Jian-min. Extraction of Genomic DNA from Corohorus olitorius L. and Establishment of SRAP Reaction System[J]. Fujian Journal of Agricultural Sciences, 2011, 26(5): 705-710.
Citation:
CHEN Hui, CHEN Mei-xia, CHEN Yan-ping, WEI Cheng-lin, LIAO Ying-min, CHEN Fu-cheng, TAO Ai-fen, XU Jian-tang, QI Jian-min. Extraction of Genomic DNA from Corohorus olitorius L. and Establishment of SRAP Reaction System[J]. Fujian Journal of Agricultural Sciences, 2011, 26(5): 705-710.
CHEN Hui, CHEN Mei-xia, CHEN Yan-ping, WEI Cheng-lin, LIAO Ying-min, CHEN Fu-cheng, TAO Ai-fen, XU Jian-tang, QI Jian-min. Extraction of Genomic DNA from Corohorus olitorius L. and Establishment of SRAP Reaction System[J]. Fujian Journal of Agricultural Sciences, 2011, 26(5): 705-710.
Citation:
CHEN Hui, CHEN Mei-xia, CHEN Yan-ping, WEI Cheng-lin, LIAO Ying-min, CHEN Fu-cheng, TAO Ai-fen, XU Jian-tang, QI Jian-min. Extraction of Genomic DNA from Corohorus olitorius L. and Establishment of SRAP Reaction System[J]. Fujian Journal of Agricultural Sciences, 2011, 26(5): 705-710.
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Dongshan Entry-Exit Inspection and Quarantine Bureau, Zhangzhou, Fujian 363401, China;
3.
Ningde Normal University Biological Engineering, Ningde, Fujian 352100, China;
4.
Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
In this study, extract high quality genomic DNA from jute (Corohorus olitorius L) and establish a stable SRAP reaction system, including template DNA concentration, Mg2+ concentration, Taq DNA polymerase concentration, dNTPs concentration and forward and reverse primer concentration, using a population F2 progeny derived from a cross of glycanes thesia (wild species) and wild leaf jute (cultivated species). The results showed that the DNA isolated with modified CTAB method had good quality. The optimum SRAP reaction system could amplify high levels of polymorphism, good repeatability and clear band pattern. SRAP-PCR system (total volume of 25 l) was established as follows: template DNA 100 ng, forward primer 0.48 molL-1, reverse primer 0.48 molL-1, Mg2+ 2.8 mmolL-1, dNTPs 0.35 mmolL-1, Taq DNA polymerase 0.7 U. It showed that the optimized system can be applied in the SRAP analysis for Corohorus olitorius L., which provided technical support for the genetic linkage map construction in the future.
LIN Z X, ZHANG X L, NIE Y C, et al. Construction of a genetic linkage map for cotton based on SRAP [J]. Chinese Science Bulletin,2003, 48(19): 2063-2067.
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