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Volume 25 Issue 6
Nov.  2011
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Article Contents
YU Zhi-xiong, CHEN Ming-xian, YUAN Ya-fang, WU Qiong-feng. Optimization of ISSR reaetion system in pitaya[J]. Fujian Journal of Agricultural Sciences, 2010, 25(6): 711-715.
Citation: YU Zhi-xiong, CHEN Ming-xian, YUAN Ya-fang, WU Qiong-feng. Optimization of ISSR reaetion system in pitaya[J]. Fujian Journal of Agricultural Sciences, 2010, 25(6): 711-715.

Optimization of ISSR reaetion system in pitaya

  • Received Date: 2010-08-23
  • Rev Recd Date: 2010-10-22
  • Publish Date: 2010-12-15
  • In order to establish a stable ISSR-PCR reaction system for pitaya,a single factor experiment and orthogonal method was applied for the optimization.The results showed that the optimum concentrations of different components in 20 μL reaction system were 2.0 μL 10×Buffer(with Mg2+),20.0 ng template DNA,0.30 mmolL-1 dNTP,0.35 μmolL-1 primer and 1.5 U Taq DNA polymerase.The optimized annealing temperature was 54.0℃ for the primer(AC)8T.The established ISSR-PCR system was tested on 6 pitaya samples with satisfactory performance.
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    齐清琳.不同品种火龙果引种栽培比较实验[J].福建林业科技,2004,31(4):48-50.
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