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Volume 25 Issue 6
Nov.  2011
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Article Contents
WANG Long-bai, ZHUANG Xiang-sheng, WEI Hong, CHE Yong-liang, CHEN Ru-jing, WU Xue-min, ZHOU Lun-jiang. Isolation and gene sequence analysis of PRRSV-FJ07A strain's structural proteins[J]. Fujian Journal of Agricultural Sciences, 2010, 25(6): 667-672.
Citation: WANG Long-bai, ZHUANG Xiang-sheng, WEI Hong, CHE Yong-liang, CHEN Ru-jing, WU Xue-min, ZHOU Lun-jiang. Isolation and gene sequence analysis of PRRSV-FJ07A strain's structural proteins[J]. Fujian Journal of Agricultural Sciences, 2010, 25(6): 667-672.

Isolation and gene sequence analysis of PRRSV-FJ07A strain's structural proteins

  • Received Date: 2010-08-30
  • Rev Recd Date: 2010-11-23
  • Publish Date: 2010-12-15
  • PRRSV-FJ07A strain was isolated and identified.The virus was tested on the Marc-145 cell and subjected to RT-PCR,TCID50,gene sequencing of structural protein and an animal challenge test.The results showed that PRRSV-FJ07A strain belonged to the family of the North American genotype of PRRSV.Its TCID50 was 10-6.5.ml-1.Compared with VR-2332 strain,the homogeneities of nucleotide and amino acid were 89.2%-95.8% and 88.1%-96.2%,respectively,90.1%-99.7% and 88.3%-100.0%,respectively,compared with HUN4 strain,and 63.7%-70.0% and 59.6%-78.9%,respectively,compared to and LV strain.After 3 to 7 days PRRSV-FJ07A innoculation,the 35-day-old piglets showed negative serum antibody of PRRSV and appeared with clinical syndromes of PRRSV infection.The gene sequence of PRRSV-FJ07A has been submitted to Genbank issued with the number of GI: 283100314.
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  • [1]
    WELCH S K,JOLIER,PEARCE D S,et al.Construction and evaluation of genetically engineered replication-defective porcine reproductive and respiratory syndrome virus vaccine candidates[J].Vet Immunol Immunopathol,2004,102 (3):277-290.
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