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Volume 25 Issue 5
Nov.  2011
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Article Contents
LI Ying, QIU Hong-duan, GONG Hui, XIE Hang, XU Bin-fu, LIU Xiao-dong. Optimization of Edwardsiella tarda Fermentation to increase biomass[J]. Fujian Journal of Agricultural Sciences, 2010, 25(5): 541-547.
Citation: LI Ying, QIU Hong-duan, GONG Hui, XIE Hang, XU Bin-fu, LIU Xiao-dong. Optimization of Edwardsiella tarda Fermentation to increase biomass[J]. Fujian Journal of Agricultural Sciences, 2010, 25(5): 541-547.

Optimization of Edwardsiella tarda Fermentation to increase biomass

  • Received Date: 2010-08-27
  • Rev Recd Date: 2010-10-09
  • Publish Date: 2010-10-15
  • Using tryptone soya broth medium,the culture conditions of Edwardsiella tarda(ETY) were optimized by a single factor and an orthogonal test to enhance the cell concentration.The results showed that the optimized medium contained 3.0 g of glucose,10.0 g of tryptone,10.0 g of peptone,2.50 g of K2HPO4 and 5.0 g of NaCl per liter.And,the most favorable fermentation conditions were at 25℃,with an initial pH of 7.5,on a stirring speed of 200 rmin-1 and with the inoculating ratio of 8%.Compared with the control,the cell concentration of ETY could be improved from 5.200×109cfumL-1 to 1.575×1010cfumL-1,i.e.,3 times increase.At the same time,the cost of liquid medium was reduced by 41.36% compared to the conventional formulation.
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  • [2] 龚艳清,陈信忠。牛蛙感染迟缓爱德华氏菌的研究[J].福建 水产,2002,2:61-64.
    [4] 董传甫,林天龙,陈日升,等。日本鳗鲡败血腹水病病原研究 [J].水产科,2002,21(1):5-8.
    [8] 肖鹏。鱼类疫苗的研究:Ⅰ:海水鱼类病原菌乳化口服疫苗的 研究;Ⅱ:迟缓爱德华氏菌和嗜水气单胞菌外膜蛋白疫苗的研 究[D].北京:中国科学院研究生院(海洋研究所),2008.
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