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Volume 25 Issue 4
Nov.  2011
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Article Contents
WAN Chun-he, LIN Su, SHI Shao-hua, LIN Qiu-min, CHEN Hong-mei, CHENG Long-fei, FU Guang-hua, LIN Fang, LIN Jian-sheng, HUANG Yu. SYBR GreenⅠ RT-PCR for rapid diagnosis of H9 Subtype Avian Influenza Virus[J]. Fujian Journal of Agricultural Sciences, 2010, 25(4): 387-391.
Citation: WAN Chun-he, LIN Su, SHI Shao-hua, LIN Qiu-min, CHEN Hong-mei, CHENG Long-fei, FU Guang-hua, LIN Fang, LIN Jian-sheng, HUANG Yu. SYBR GreenⅠ RT-PCR for rapid diagnosis of H9 Subtype Avian Influenza Virus[J]. Fujian Journal of Agricultural Sciences, 2010, 25(4): 387-391.

SYBR GreenⅠ RT-PCR for rapid diagnosis of H9 Subtype Avian Influenza Virus

  • Received Date: 2010-06-28
  • Rev Recd Date: 2010-08-16
  • Publish Date: 2010-08-15
  • A pair of specific primers targeting hemagglutinin gene of H9 avian influenza virus(AIV) was designed for the development of a SYBR Green I fluorescent based real-time RT-PCR(RRT-PCR) to quantify the H9 subtype AIV for rapid diagnosis.The detection limit of RRT-PCR was 8.33×102 plasmid copies.The melting curve analysis using SYBR Green I dye showed one specific peak at the melting temperature of(83.83±0.22)℃ with no primer-dimers peak.No amplification was detected by this method from the unrelated RNA samples,such as H5 subtype AIV,duck hepatitis virus,duck plague virus or avian paramyxovirus type-1.High reproducibility was obtained for detecting plasmid DNA with intra-assay of 0.52%-1.48% and inter-assay of 0.71%-2.21%.
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    PEIRIS M,YUEN K,LEUNG C,et al.Human infection with influenza H9N2[J].Lancet,1999,354(9182):916-917.
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    万春和,傅光华,程龙飞,等.A/Chicken/Zhejiang/HJ/2007(H9N2) 禽流感病毒全基因测序及遗传进化分析[J].农业生物技术学报,2009,17(5):750-757.
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