Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.
Citation:
LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.
LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.
Citation:
LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.
Avian leukosis is one of the main chicken tumor diseases.It causes considerable economic loss for poultry farmers.A new,rapid diagnostic mehtod applying the real-time florescence quantitative polymerase chain reaction(PT-PCR)was developed for detecting the avian leukosis virus subgroup J(ALV-J).A set of subgroup J special primers for gp85 gene was used.Validations on repeatability,specificity and sensitivity of the methodology showed its potential applicability for clinical diagnosis of ALV-J.
BAGUST T J,GRIMES T M,DENNETT D P.Infection studies on a reticuloendotheliosis virus contaminant of a commercial Marek′s disease vaccine[J].Aust Vet J,1979,55:153-157.
BAI J,HOWES K,PAYNE L N,et al.Sequence of host-range determinants in the env gene of a full-length,infectious proviral clone of exogenous avian leukosis virus HPRS-103 confirms that it represents a new subgroup(designated J)[J].Journal of General Virology,1995,76:181-187.
[4]
SMITH L M,TOYE K,HOWES N,et al.Novel endogenous retroviral sequences in the chicken genome closely related to HPRS-103(subgroup J)avian leukosis virus[J].J Gen Virol,1999,80:261-268.