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Volume 25 Issue 3
Nov.  2011
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Article Contents
LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.
Citation: LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.

Quantitative detection of ALV-J by fluorescence RT-PCR

  • Received Date: 2010-03-03
  • Rev Recd Date: 2010-04-07
  • Publish Date: 2010-06-15
  • Avian leukosis is one of the main chicken tumor diseases.It causes considerable economic loss for poultry farmers.A new,rapid diagnostic mehtod applying the real-time florescence quantitative polymerase chain reaction(PT-PCR)was developed for detecting the avian leukosis virus subgroup J(ALV-J).A set of subgroup J special primers for gp85 gene was used.Validations on repeatability,specificity and sensitivity of the methodology showed its potential applicability for clinical diagnosis of ALV-J.
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    BAI J,HOWES K,PAYNE L N,et al.Sequence of host-range determinants in the env gene of a full-length,infectious proviral clone of exogenous avian leukosis virus HPRS-103 confirms that it represents a new subgroup(designated J)[J].Journal of General Virology,1995,76:181-187.
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    SMITH L M,TOYE K,HOWES N,et al.Novel endogenous retroviral sequences in the chicken genome closely related to HPRS-103(subgroup J)avian leukosis virus[J].J Gen Virol,1999,80:261-268.
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    张立成,关云涛,陈洪岩,等.应用PT-PCR技术检测禽白血病病毒及其在不同组织中检出结果的比较[J].中国实验动物学杂志,2002,12(5):303-304.
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    童淑梅,杨玉莹,赵振华,等.鸡内源性类J亚群禽白血病病毒gp85基因的序列分析[J].动物医学进展,2007,28(1):17-21.
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