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ISSR markers were applied to detect the genetic diversities of 47 cultivars of Liriope muscari(Decn.)Bailey.One hundred thirty one bands were amplified with 18 primers,including 83 polymorphic bands.The percentage of the polymorphic bands was 63.4%.By cluster analysis based on ISSR markers using UPGMA,the 47 cultivars were classified into 4 groups.The genetic similarity was from 0.64 to 0.98.As a group,the wild species from Putian had the greatest genetic difference from the landrace.Determined by the cluster analysis,the geographical distribution did not seem to be distinctive.The genetic diversity of L. muscari Bailey appeared to be high,based on our observation.This could provide references for analyzing genetic relationship and utilizing the genetic diversity for agricultural applications.