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Volume 25 Issue 3
Nov.  2011
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OUYANG Sui-dong, LIN Feng-qiang, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, ZHU Xiao-li, HU Qi-lin, CHEN Shao-ying. σC gene expresson of muscovy duck reovirus MW9710 strain in Pichia pastoris[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 241-244.
Citation: OUYANG Sui-dong, LIN Feng-qiang, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, ZHU Xiao-li, HU Qi-lin, CHEN Shao-ying. σC gene expresson of muscovy duck reovirus MW9710 strain in Pichia pastoris[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 241-244.

σC gene expresson of muscovy duck reovirus MW9710 strain in Pichia pastoris

  • Received Date: 2010-02-03
  • Rev Recd Date: 2010-05-17
  • Publish Date: 2010-06-15
  • The σC gene of DRVMW9710 was inserted into pPIC9K to construct a recombinant secretory vector,pPIC9K-σC.Then,pPIC9K-σC was transformed into E. coli DH5α.The recombinant vector was identified by PCR,enzymatic analysis and sequencing.The positive plasmid was linearized by Sac I and transformed into GS115 by electroporation.The insert was integrated into genomic DNA of Pichia pastoris by means of homologous recombination.The recombinants with multiple integrated copies of σC were screened using gradient concentration G418,and inducted with methanol.The results of SDS-PAGE and Western-blot showed that the highly expressed protein had an immunoreaction property.
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  • [1]
    欧阳岁东,胡奇林,林锋强,等.番鸭呼肠孤病毒MW9710株δC蛋白基因的克隆和序列分析[J].中国预防兽医学报,2006,28(5):510-513.
    [2]
    GALL-RECULE G L,CHERBONNEL M.Molecular characterization and expression of the S3 gene of muscovy duck reovirus strain 89026[J].J Gen Girology,1999,80:195-203.
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