• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 25 Issue 2
Nov.  2011
Turn off MathJax
Article Contents
LIN Zong-keng, WEN Qing-fang, LIN Yi-zhang. Technology system of culture for asparagus officiralis L. anther[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 212-216.
Citation: LIN Zong-keng, WEN Qing-fang, LIN Yi-zhang. Technology system of culture for asparagus officiralis L. anther[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 212-216.

Technology system of culture for asparagus officiralis L. anther

  • Received Date: 2009-10-28
  • Rev Recd Date: 2010-01-18
  • Publish Date: 2010-04-15
  • Experiments were conducted to study the callus inducement and green shoot differentiation of the anther of the breed "Gijnlim",Asparagus officinalis L. The results showed that the optimum asparagus callus induction could be obtained by inoculation on a 1/2 MS culture medium containing 2.0 mg NAAL-1,1.0 mg 6-BAL-1and 6% sucrose after storage at 4℃ for 5-7 days. Moreover,a higher induction rate could be accomplished with a 32℃ incubation for 5 days following the inoculation. Supplementation with 300 mg hydrolyzed caseinL-1in the MS medium also benefited the callus induction. Callus type and age affected the regeneration rate. The regeneration rate of the callus of the dense form was higher than that of the loose form. On the different callus ages (i.e.,10-50 days),30-to 40-day-olds had the highest green shoots regeneration rate. The microscopic examination showed that,among the green shoots from the loose callus,5.7% of the plants were haploids,65.1% diploids,11.9% tetraploids and 17.3% aneuploids; while among the green shoots from the dense callus,28.4% were haploids,45.3% diploids,9.6% tetraploids and 16.7% aneuploids; and,the haploids from the dense callus were more than those from the loose callus.
  • loading
  • [1]
    严仁玲,张磊,张存金,等.石刁柏花药离体培养及单倍体植株再生的研究[J].华北农学报,1992,7(1):75-82.
    [2]
    李守岭,庄南生.植物花药培养及影响因素研究进展[J].亚热带植物科学,2006,35(3):76-80.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2279) PDF downloads(737) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return