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Volume 25 Issue 2
Nov.  2011
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Article Contents
WU Yu-fen, ZHOU Xian-zhi, CHEN Sheng, CHEN Yang, ZHAO Yi-jie. Phylogeny of Cucurbita Based on ITS sequence data[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 170-175.
Citation: WU Yu-fen, ZHOU Xian-zhi, CHEN Sheng, CHEN Yang, ZHAO Yi-jie. Phylogeny of Cucurbita Based on ITS sequence data[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 170-175.

Phylogeny of Cucurbita Based on ITS sequence data

  • Received Date: 2010-01-05
  • Rev Recd Date: 2010-04-01
  • Publish Date: 2010-04-15
  • The relationships and differences among the species,Cucurbita,pepo and ficifofia (Cucurbitaceae:Cucurbita),were examined using data from the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA. The ITS sequences were obtained from Cucurbita,pepo and ficifofia 3 species. The results showed that,within Cucurbita,ITS1 was 188-191bp (C. Cucurbita,C. pepo and C. ficifofia) and ITS2 ranged from 251-263bp (C. Cucurbita,C. pepo and C. ficifofia). There were 33 variable positions (17.55%) and 24 informative sites (12.77%) within Cucurbita found in the ITS1 sequences,while 45 variable positions (17.93%) and 31 informative sites (12.35%) found in the ITS2 sequences.The G+C content of ITS1 was 51.60% to 54.50% and that of ITS2 ranged from 53.78% to 56.98%.The resulting trees from the ITS1 and ITS2 sequences showed that the relationship between C. Cucurbita and C. pepo was closer than that between C. Cucurbita and C. ficifofia.
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