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Volume 25 Issue 2
Nov.  2011
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Article Contents
YE Yao-hui, WU Nan-yang, HUANG Mei-qing, ZHENG Min, LEI Song-bo, ZHANG Shi-zhong. Pathogenic detection and identification of swine respiratory syndrome[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 131-134.
Citation: YE Yao-hui, WU Nan-yang, HUANG Mei-qing, ZHENG Min, LEI Song-bo, ZHANG Shi-zhong. Pathogenic detection and identification of swine respiratory syndrome[J]. Fujian Journal of Agricultural Sciences, 2010, 25(2): 131-134.

Pathogenic detection and identification of swine respiratory syndrome

  • Received Date: 2010-01-05
  • Rev Recd Date: 2010-03-16
  • Publish Date: 2010-04-15
  • The study detected various kinds of pathogens in pigs suffering from respiratory syndrome by PCR technique and immunofluorescent test. The RT-PCR results showed that the tissue samples of lung and lymph nodes from diseased pigs were positive for PRRSV variant but negative for PRV,CSFV,PCV2 or SIV. A virus strains were also isolated from positive samples by inoculating it onto Marc-145. The 30 days-old piglets inoculated with the isolates showed the same symptoms as appeared naturally on the diseased pigs in the clinic. The virus was recovered from and the interstitial Pneumonia observed in the diseased pigs. Thus,it was concluded that the respiratory disease outbreak in the swine farm was caused by a PRRSV variant.
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    车勇良,王隆柏,魏宏,等.PCR技术在猪圆环病毒2型检测中的应用[J].动物医学进展,2006,27(6):70-80,102.
    [2]
    郝晓芳,周艳君,田志军,等.高致病性猪繁殖与呼吸综合征病毒RT-PCR鉴别诊断方法的建立[J].中国预防兽医学报,2007,29(9):704-709.
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