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WANG Shao, CHEN Shao-ying, ZHU Xiao-li, CHENG Xiao-xia, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Determination and analysis of complete genomic sequence of duck hepatitis virus NA strain[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 18-22.
Citation:
WANG Shao, CHEN Shao-ying, ZHU Xiao-li, CHENG Xiao-xia, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Determination and analysis of complete genomic sequence of duck hepatitis virus NA strain[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 18-22.
WANG Shao, CHEN Shao-ying, ZHU Xiao-li, CHENG Xiao-xia, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Determination and analysis of complete genomic sequence of duck hepatitis virus NA strain[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 18-22.
Citation:
WANG Shao, CHEN Shao-ying, ZHU Xiao-li, CHENG Xiao-xia, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Determination and analysis of complete genomic sequence of duck hepatitis virus NA strain[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 18-22.
The cDNA fragments of DHV-NA were amplified with RT-PCR.Complete genomic sequence was determined and phylogenetic tree was analyzed.The complete genome of DHV-NA was determined to consist of 7692 nucleotides,except Poly(A) tail.The lengths of 5′and 3′uncoding regions were 626 and 316 nucleotides,respectively.There was a single open reading frame(ORF,627-7376nt) encoding 2249 amino acids.Compared to the other DHV-Ⅰstrains(i.e.,R85952,H,DRL-62,C80,03D,JX),the nucleotide sequence homology was 94.3%-96.9% and the amino acids sequence homology was 97.8%-98.8%.Compared to NDHV-90D strain,the homologies of the nucleotide sequence and amino acid sequence were 72.6% and 82.3%,respectively.The results indicated that the phylogenetic homology between DHV-NA and other DHV-Ⅰstrains was higher than that between DHV-NA and N-DHV 90D strain.
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