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HUANG Yu-ji, CHEN Jing-ying, SU Hai-lan, HUANG Ying-zhen, WAN Xue-feng. rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 508-512.
Citation:
HUANG Yu-ji, CHEN Jing-ying, SU Hai-lan, HUANG Ying-zhen, WAN Xue-feng. rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 508-512.
HUANG Yu-ji, CHEN Jing-ying, SU Hai-lan, HUANG Ying-zhen, WAN Xue-feng. rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 508-512.
Citation:
HUANG Yu-ji, CHEN Jing-ying, SU Hai-lan, HUANG Ying-zhen, WAN Xue-feng. rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions[J]. Fujian Journal of Agricultural Sciences, 2009, 24(6): 508-512.
The 18S-26S rDNA-ITS base sequences of Radix ophiopogonis and Radix liriopes from different geographical regions were compared by using the specific PCR-ITS.The complete sequences of ITS and 5.8S rDNA,as well as partial sequences of 18S rDNA and 26S rDNA,in the range of 696-711 bp for the seven samples were obtained.By comparing the sequences,it was apparent that the ITS sequences of R. ophiopogonis and R. liriopes from different regions varied.Our analytical results provided a basis for molecular identification of the species.