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Volume 24 Issue 5
Nov.  2011
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WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, ZHU Xiao-li, CHEN Shi-long, OUYANG Sui-dong. Expression ofσC gene of muscovy duck reovirus,MW9710 strain,by using pMAL-p2X system[J]. Fujian Journal of Agricultural Sciences, 2009, 24(5): 396-399.
Citation: WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, ZHU Xiao-li, CHEN Shi-long, OUYANG Sui-dong. Expression ofσC gene of muscovy duck reovirus,MW9710 strain,by using pMAL-p2X system[J]. Fujian Journal of Agricultural Sciences, 2009, 24(5): 396-399.

Expression ofσC gene of muscovy duck reovirus,MW9710 strain,by using pMAL-p2X system

  • Received Date: 2009-09-09
  • Rev Recd Date: 2009-09-30
  • Publish Date: 2009-10-15
  • To obtain recombinant Muscovy duck reovirus(MDRV) _σC protein by prokaryotic expression for further study on its functions,the encoding sequence of the _σC gene from the MDRV MW9710 strain was amplified with RT-PCR and inserted into pMAL-p2X vector to establish the prokaryotic expression plasmid.After transforming the plasmid,pMAL-p2X-_σC,into E.coil BL21(DE3),the bacteria were induced by IPTG.The protein was expressed efficiently in both soluble and insoluble forms,and was positively recognized by the reference serum.The SDS-PAGE and western-blot analysis showed the recombinant pMAL-p2X-_σC produced had an apparent molecular weight of 72KDa.The soluble _σC protein was purified and obtained using MBP-affinity chromatography.The result provided a foundation for the gene’s epitope identification and development of a clinical diagnostic kit in the future.
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