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Volume 24 Issue 3
Nov.  2011
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DONG Xiao-ning, ZHANG Xiao-pei, LI Wen-yang. Genetic diversity of 18 ryegrass varieties determined by RAPD analysis[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 266-269.
Citation: DONG Xiao-ning, ZHANG Xiao-pei, LI Wen-yang. Genetic diversity of 18 ryegrass varieties determined by RAPD analysis[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 266-269.

Genetic diversity of 18 ryegrass varieties determined by RAPD analysis

  • Received Date: 2009-04-15
  • Rev Recd Date: 2009-05-15
  • Publish Date: 2009-06-15
  • RAPD molecular-marker technique was used to detect the genetic diversity among 18 ryegrass germplasms.Their genomic DNA were tested with RAPD using 26 10-mer primers screened from 110 arbitrary 10-mer primers.A total of 197 RAPD bands were obtained,in which 187 were polymorphic.The average percentage of polymorphic bands was 94.9%.The variation range of genetic distances was from 0 to 0.846 7.By a UPGMA cluster analysis on the RAPD molecular-markers,these ryegrass varieties were divided into three groups:(1) Yatsyn,Tetragold,Pandora,Abundant,Angus,Italian Ryegrass,Barwoltra,Normal Annual Ryegrass,Green Spirit,Normal Diploid and Triticale;(2) Peak,Cailente,Pinnacle,Primer and Bright Star;and(3) Dong Mu 70.
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    黄艳霞,白昌军.分子标记技术及在牧草中的应用[J].热带农业科学,2008,28(4):81-85.
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