Analysis of disease and insect resistance genes in excellent sterile line FunongA

LIAN Ling; TU Shihang; ZHANG Junian; DONG Meng; HE Wei; ZHENG Yanmei; XIE Zhenxing; SHI Longqing; JIANG Zhaowei; WU Chunzhu

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LIAN L, TU S H, ZHANG J N, et al. Analysis of disease and insect resistance genes in excellent sterile line FunongA [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−10

Citation:

LIAN L, TU S H, ZHANG J N, et al. Analysis of disease and insect resistance genes in excellent sterile line FunongA [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−10

LIAN L, TU S H, ZHANG J N, et al. Analysis of disease and insect resistance genes in excellent sterile line FunongA [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−10

Citation:

LIAN L, TU S H, ZHANG J N, et al. Analysis of disease and insect resistance genes in excellent sterile line FunongA [J]. Fujian Journal of Agricultural Sciences，2024，39（9）：1−10

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Analysis of disease and insect resistance genes in excellent sterile line FunongA

LIAN Ling^{1, 2
,},
TU Shihang¹,
ZHANG Junian¹,
DONG Meng¹,
HE Wei^{1, 2},
ZHENG Yanmei^{1, 2},
XIE Zhenxing¹,
SHI Longqing¹,
JIANG Zhaowei¹,
WU Chunzhu^{1
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350019, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, China/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, Fujian　350003, China

Received Date: 2024-06-05
Rev Recd Date: 2024-07-16

Available Online: 2024-10-31

Abstract

Abstract

Objective Analysis of disease and insect resistance genes in FunongA, a three-line indica cytoplasmic male sterile line, will provide an important theoretical basis for the application of the sterile line in breeding production. Method Genomic DNA of FunongA, FudaoB and Huahangsimiao was extracted by CTAB method, then blast resistance genes were detected with molecular markers. Resistance genes related to bacterial blight, viral diseases and brown planthopper were analyzed by high density chip detection. Blast resistance genes of FunongA, Huahangsimiao and FudaoB were obtained by PCR, then sequencing and comparative analysis were performed. The samples were collected at 0h, 24h, 48h, 72h and 96h after inoculation with blast fungus. Total RNA was extracted using the Trizol method, and the expressions of blast resistance genes analyzed by SYBR Green I fluorescent quantitative PCR (qRT-PCR). Result FunongA, FudaoB and Huahangsimiao contain blast resistance genes Pib, Pia, Pid3, Pid2, bacterial blight resistance gene Xa21 and yellow mottle virus resistance gene Rymv1. Furthermore, FunongA and Huahangsimiao contain blast resistance gene Pi5 and stripe virus resistance gene STV11, and FudaoB contain blast resistance genes Pita and Pi37. In addition, they do not contain brown planthopper resistance genes. The fragments of Pi5, Pia, Pib, Pid3, Pid2 were 5672 bp, 2597 bp, 5532 bp, 2865 bp, 3847 bp, respectively. The sequences of Pi5, Pid3 and Pid2 in FunongA were consistent with that in Huahangsimiao, while there were 2 and 5 bases different from Pid3 and Pid2 of Fudao B, respectively. In Funong A, the expression of rice blast resistance genes Pi5 and Pib was significantly induced by blast fungus, and the highest at 72 h after inoculation. The expression of Pia and Pid3 increased gradually with the prolongation of inoculation time, and the highest at 96 h. The expression of Pid2 increased firstly and then decreased, and the highest at 24 h. Conclusion Three-line indica cytoplasmic male sterile line FunongA contains blast resistance genes Pi5, Pib, Pia, Pid3 and Pid2, which significantly induced by blast fungus. Funong A also contains bacterial blight resistance gene Xa21, yellow mottle virus resistance gene Rymv1 and stripe virus resistance gene STV11.
- FunongA,
- blast resistance gene,
- sequential analysis,
- gene expression

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References(22)

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