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Volume 24 Issue 1
Nov.  2011
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SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, YANG Wei-xing, HUANG Yu. Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 11-13.
Citation: SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, YANG Wei-xing, HUANG Yu. Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 11-13.

Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1

  • Received Date: 2008-12-09
  • Rev Recd Date: 2009-01-29
  • Publish Date: 2009-02-15
  • An RT-PCR method was developed to detect virulent strains of waterfowl-origin avian paramyxovirus type 1(vwAPMV-1).The primers were designed according to the sequences of the fusion protein gene of vwAPMV-1s available at the GenBank.The result showed that the 400 bp of specific fragments were amplified in vwAPMV-1.However,negative results were obtained from Pasteurella multocide,EDS,DEV,IBDV,H9N2 influenza virus,DHV-1 and low virulent strains of waterfowl-origin avian paramyxovirus type 1.And,the 10pg of vwAPMV-1 RNA was amplified by the sensitivity test for the RT-PCR.The results indicated that the RT-PCR could be used for vwAPMV-1 detection.
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