Multiplex-PCR analyses and seasonal changes of virulence genes of <i>E.coli</i> isolated from Min river watersheds in Fuzhou

HUANG Wen-wen; CHEN Bin; ZHUANG Yi-ting; CHEN Ning; ZHENG Wei-wen

Volume 23 Issue 4

Nov. 2011

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Article Navigation > Fujian Journal of Agricultural Sciences > 2008 > 23(4): 408-414

HUANG Wen-wen, CHEN Bin, ZHUANG Yi-ting, CHEN Ning, ZHENG Wei-wen. Multiplex-PCR analyses and seasonal changes of virulence genes of E.coli isolated from Min river watersheds in Fuzhou[J]. Fujian Journal of Agricultural Sciences, 2008, 23(4): 408-414.

Citation:

HUANG Wen-wen, CHEN Bin, ZHUANG Yi-ting, CHEN Ning, ZHENG Wei-wen. Multiplex-PCR analyses and seasonal changes of virulence genes of E.coli isolated from Min river watersheds in Fuzhou[J]. Fujian Journal of Agricultural Sciences, 2008, 23(4): 408-414.

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Multiplex-PCR analyses and seasonal changes of virulence genes of E.coli isolated from Min river watersheds in Fuzhou

1.
College of Life Science, Fujian Normal University, Fuzhou, Fujian 350108, China;
2.
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
3.
Fuzhou Environmental Monitoring Central Station, Fuzhou, Fujian 350003, China;
4.
Fujian Environment Protection Bureau, Fuzhou, Fujian 350003, China;
5.
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2008-07-03
Rev Recd Date: 2008-09-25
Publish Date: 2008-10-15

Abstract

Abstract

Toxic genes of the potential pathogenic E.coli collected in different seasons from Min River watersheds in Fuzhou were analyzed using Multiplex-PCR.The results indicated that 840 out of 2015 E.coli isolates were potential pathogens classified as EHEC,EAEC,EPEC and ETEC groups.Ten virulence genes were detected with dominant genes,aidA-1,astA and sepA.The numbers of bacteria,E.coli and E.coli carrying virulence genes,increased in the spring and summer,peaked in the fall,and decreased in the winter.
- Min River,
- E.coli,
- virulence gene,
- multiplex-PCR

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References(3)

References

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