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Institute of Animal Husbandry and Veterinary Medicine, FuJian Academy of Agricultural Sciences, Fujian Animal Disease Control Technology Development Center, Fuzhou Fujian 350013, China;
2.
Fujian Entry-exit Inspection and Quarantine Inspection and Quarantine Technology Centr, Fuzhou, Fujian 350003, China;
3.
College of Animal Science, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China
To fully understand the knowledge of variant porcine reproductive and respiratory syndrome virus (PRRSV) genetic variation and pathogenicity, the ORF5 and NSP2 genomes of PRRSV-FJ09A strain were amplified by RT-PCR and sequenced. Comparing with PRRSV-VR2332, ORF5 gene shared 87.6%of nucleotide homologies and 88.1% of the deduced amino acid homologies, there were point mutations within deduced amino acids, focus on amino acids of associated with virulence, epitope and N-glycosylation sites;NSP2 gene shared 80.9% of nucleotide homologies and 77.0% of the deduced amino acid homologies, and it shared a common feature of 31 amino acids deletion in three places which one place more of a phenylalanine(F) than other local reports. Comparing these sequences with Chinese PRRSV strains published after 2006,ORF5 gene shared 98.5%-99.7% of nucleotide homologies and 97.5%-99.5% of the deduced amino acid homologies, NSP2 gene shared 98.4%-99.3% of nucleotide homologies and 97.3%-98.9% of the deduced amino acid homologies. The phylogeneic tree revealed PRRSV-FJ09A strain isolated had closer relationship with PRRSV-FJ06A strain. According to the animal experiment PRRSV-FJ09A had a high pathogenicity to 30 d pigs.
ZHOU L, ZHONG J L, ZENG J W, et al. The 30-Amino-Acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence [J]. J Virol, 2009, 83(10):5156-5167.
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