• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 27 Issue 10
Dec.  2012
Turn off MathJax
Article Contents
FU Guang-hua, CHEN Hong-mei, HUANG Yu, WAN Chun-he, FU Qiu-ling, CHENG Long-fei, SHI Shao-hua, LIN Fang, LIN Jian-sheng. Identification and Sequence Analysis of Tembusu Virus Isolated from White-feather Mule Ducks[J]. Fujian Journal of Agricultural Sciences, 2012, 27(10): 1027-1031.
Citation: FU Guang-hua, CHEN Hong-mei, HUANG Yu, WAN Chun-he, FU Qiu-ling, CHENG Long-fei, SHI Shao-hua, LIN Fang, LIN Jian-sheng. Identification and Sequence Analysis of Tembusu Virus Isolated from White-feather Mule Ducks[J]. Fujian Journal of Agricultural Sciences, 2012, 27(10): 1027-1031.

Identification and Sequence Analysis of Tembusu Virus Isolated from White-feather Mule Ducks

  • Received Date: 2012-09-07
  • Rev Recd Date: 2012-10-11
  • Publish Date: 2012-12-18
  • A viral isolate (named as ZZ40) was abtained from liver of white-feather mule ducks with irregular hemorrhagic hepatitis, and were identified as duck tembus virus (DTMUV) by RT-PCR. The virus was specifically neutralized by anti-DTMUV hyperimmune serum. Gene fregments were abtained by using three sets of primers aimed at viral structural genes, and shared no less than 99.1% nucleotide sequence identity with those of avian tembus viruses isolated recently, and no more than 88.6% with that of Mosquito-borne tembus virus MM-1775 strain. Phylogenetic analysis showed that ZZ40 strain clustal into the same clade with those duck viruses isolated recently. These results indicated that the white-feather mule ducks in this case was infected with duck tembusu virus, which has no abviously variation in antigenicity compared with those of the early isolates of duck tembusu virus from egg-laying duck.
  • loading
  • [1]
    CAO Z, ZHANG C, LIU Y, et al. Tembusu virus in ducks, China[J].Emerg Infect Dis, 2011, 17(10): 1873-1875.
    [2]
    曹贞贞, 张存, 黄瑜, 等. 鸭出血性卵巢炎的初步研究[J]. 中国兽医杂志, 2010, 46(12):3-6.
    [3]
    傅光华, 黄瑜, 施少华, 等. 鸡黄病毒的分离与初步鉴定[J]. 福建畜牧兽医, 2011, (3):1-2.
    [4]
    PLUMMER P J, ALEFANTIS T, KAPLAN S, et al. Detection of duck enteritis virus by polymerase chain reaction[J]. Avian Disease, 1998, 42(3): 554-564.
    [5]
    GBT 16550-2008, 新城疫诊断技术[S]. 2008.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2214) PDF downloads(372) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return