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Volume 27 Issue 9
Nov.  2012
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Article Contents
CHEN Ru-jing, WU Xue-min, CHE Yong-liang, WANG Long-bai, WEI Hong, ZHUANG Xiang-sheng, LIU Yu-tao, YAN Shan, ZHOU Lun-jiang. Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 941-944.
Citation: CHEN Ru-jing, WU Xue-min, CHE Yong-liang, WANG Long-bai, WEI Hong, ZHUANG Xiang-sheng, LIU Yu-tao, YAN Shan, ZHOU Lun-jiang. Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 941-944.

Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian

  • Received Date: 2012-07-10
  • Rev Recd Date: 2012-08-02
  • Publish Date: 2012-09-30
  • The 3D gene of porcine kobuvirus was amplified by RT-PCR from tissues of piglets association with diarrhea from Fujian and then sequenced. The results showed that the porcine kobuvirus 3D gene was 1 407 bp in length, coding an open reading frame (ORF) containing 468 amino acids.Compared with the published PKV 3D genes by the bioinformatics software, the sequenced gene shared the highest homogeneity with SH-W-CHN/2010/China strain of 93.6%. In the phylogenetic tree, the porcine kobuvirus Fujian strain was at the same branch with other strains isolated in China while the Hungarian isolates share another branch, which indicated that the porcine kobuvirus had its own evolution status in different continents.
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  • [1]
    REUTER G, BOROS A, PANKOVICS P. Kobuviruses-a comprehensive review [J]. Rev Med Virol, 2011, 21(1):32-41.
    [2]
    REUTER G, BOLDIZS R A, PANKOVICS P. Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae [J]. Arch Virol, 2009, 154(1):101-108.
    [3]
    陈如敬,吴学敏,车永良,等.猪流行性腹泻病毒FJ-11A株的分离与ORF3基因序列分析[J].福建农业学报,2011,26(6):947-951.
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