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LI Jian-lin, LI Hong-xia, TANG Yong-kai, YU Ju-hua, DONG Zai-jie. Genetic Diversity Analysis of Six Populations of Common Carp by Microsatellite Markers[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 936-940.
Citation:
LI Jian-lin, LI Hong-xia, TANG Yong-kai, YU Ju-hua, DONG Zai-jie. Genetic Diversity Analysis of Six Populations of Common Carp by Microsatellite Markers[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 936-940.
LI Jian-lin, LI Hong-xia, TANG Yong-kai, YU Ju-hua, DONG Zai-jie. Genetic Diversity Analysis of Six Populations of Common Carp by Microsatellite Markers[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 936-940.
Citation:
LI Jian-lin, LI Hong-xia, TANG Yong-kai, YU Ju-hua, DONG Zai-jie. Genetic Diversity Analysis of Six Populations of Common Carp by Microsatellite Markers[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 936-940.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture/ Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China
For evaluation and rational utilization of the germplasm resources of Common carp, the genetic diversity of Cyprinus carpio L, C.carpio haematopterus, C.carpio var.wuyuanensis, C.carpio var .singuonensis、C.carpio haematopterus Temminck et Schlegfl and C.carpio var.jian were analyzed with 13 microsatellite markers. In total, 142 alleles were detected in the six populations of common carp by the 13 microsatellite loci, whose DNA fragment length ranged from 116 bps to 280 bps. The average heterozygosity observed (Ho) varied from 0.564 to 0.705, and the heterozygosity expected (He) varied from 0.611 to 0.776 respectively in the six populations. The average polymorphic information content (PIC) of the 13 loci in the 6 populations of common carp ranged from 0.573 to 0.749. The average fixation index (FIS) showed heterozygote excess only in the C.carpio var. jian population,but heterozygote deficiency in the other 5 populations. The results suggested that rich polymorphism information content , large genetic diversity and great potential for selective breeding lied in the six populations. The genetic distance between each populations of the six Common carp populations and the UPGAM dendrogram showed that C.carpio L and C.carpio var .singuonensis were the farthest in relationship, and C.carpio haematopterus Temminck et Schlegfl and C.carpio var. jian were the nearest.