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ZHANG Zhi-deng, LUO Tu-yan, RAO Qiu-hua, CAO Zhi-yun, ZHENG Teng. Glutamine Synthetase Gene Amplification by 3'RACE and Bioinformatics Analysis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 919-923.
Citation:
ZHANG Zhi-deng, LUO Tu-yan, RAO Qiu-hua, CAO Zhi-yun, ZHENG Teng. Glutamine Synthetase Gene Amplification by 3'RACE and Bioinformatics Analysis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 919-923.
ZHANG Zhi-deng, LUO Tu-yan, RAO Qiu-hua, CAO Zhi-yun, ZHENG Teng. Glutamine Synthetase Gene Amplification by 3'RACE and Bioinformatics Analysis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 919-923.
Citation:
ZHANG Zhi-deng, LUO Tu-yan, RAO Qiu-hua, CAO Zhi-yun, ZHENG Teng. Glutamine Synthetase Gene Amplification by 3'RACE and Bioinformatics Analysis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 919-923.
On the basis of GS mRNA and specific primer, Using 3'RACE PCR amplified the gene sequence and analyzed some bioinformatics information of the sequence in NCBI web. GS DNA fragment was about 1.0 kb.The sequence blast showed that the homology of fragment with GS gene of Asp.niger in GenbBank was more than 97% and with other Asp were more than 50%.Open reading frame analyzed that it included 2 exon,one of which had 100% homology with GS of Asp.niger.
MARGELIS S, D'SOUZA C, SMALL A J, et al. Role of glutamine synthetase in nitrogen metabolite repression in Aspergillus nidulans[J]. J Bacterio, 2001,183(20):5826-5833.
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