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Volume 27 Issue 9
Nov.  2012
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Article Contents
LIAN Ling, HE Wei, CAI Qiu-hua, XU Hui-bin, ZHU Yong-sheng, ZHANG Jian-fu, XIE Hua-an. Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 913-918.
Citation: LIAN Ling, HE Wei, CAI Qiu-hua, XU Hui-bin, ZHU Yong-sheng, ZHANG Jian-fu, XIE Hua-an. Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 913-918.

Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare

  • Received Date: 2012-06-01
  • Rev Recd Date: 2012-07-09
  • Publish Date: 2012-09-30
  • A cDNA encoding IPA1 was isolated from Nipponbare by RT-PCR, sequencing results showed that the sequence consists of 1 257 bp with a complete CDS and encodes a protein of 418 amino acids. In addition, the prediction results indicated that molecular weight of target protein was 42.51 kD and theoretical isoelectric point was 9.37. The plant expression vector was constructed by ligating the cDNA fragment into expression vector pHI, then the recombinant plasmid pHI-IPA1 was confirmed by restriction enzyme digestion analysis with SmaI、SacI, and further verified by DNA sequencing. Vector plasmid was transformed into Agrobacterium tumefaciens, which will lay foundation for rice genetic transformation.
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    谢华安.中国特别是福建的超级稻研究进展[J].中国稻米,2004,(2):7-9.
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    周开达,马玉清,刘太清,等.杂交水稻亚种间重穗型组合的选育——杂交水稻超高产育种的理论与实践[J].四川农业大学学报,1995,13(4): 403- 407.
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