Optimization of Two-dimensional Polyaneylamide Gel Electrophoresis for Proteome of <em>Kandelia candel</em> Roots

CHEN Wei; HUANG Rong-hui; LIN Xiao-min; YANG Zhen-hong; WENG Zhao-xia; TAN Fang-lin

Volume 27 Issue 8

Nov. 2012

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(8): 863-868

CHEN Wei, HUANG Rong-hui, LIN Xiao-min, YANG Zhen-hong, WENG Zhao-xia, TAN Fang-lin. Optimization of Two-dimensional Polyaneylamide Gel Electrophoresis for Proteome of Kandelia candel Roots[J]. Fujian Journal of Agricultural Sciences, 2012, 27(8): 863-868.

Citation:

CHEN Wei, HUANG Rong-hui, LIN Xiao-min, YANG Zhen-hong, WENG Zhao-xia, TAN Fang-lin. Optimization of Two-dimensional Polyaneylamide Gel Electrophoresis for Proteome of Kandelia candel Roots[J]. Fujian Journal of Agricultural Sciences, 2012, 27(8): 863-868.

Citation:

PDF( 1687 KB)

Optimization of Two-dimensional Polyaneylamide Gel Electrophoresis for Proteome of Kandelia candel Roots

1.
School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Fujian Academy of Forestry, Fuzhou, Fujian 350002, China

Received Date: 2012-05-28
Rev Recd Date: 2012-07-28
Publish Date: 2012-08-30

Abstract

Abstract

A two-dimensional polyarelamide gel electrophoresis (2D-PAGE) system was established for proteomic analysis of Kandelia candel roots. Protein extraction, dissolution, loading quantity, IEF and SDS-PAGE parameters in the two-dimensional electrophoresis (2-DE) were optimized. The results showed that phenol method was suitable for the root protein extraction, the protein isoelectric points mainly distributed between pH 4 and 7. The lysis buffer with 2 mmol稬-1 thiourea improved dissolution of protein effectively and the resolution of spectrum for the dissolved protein was increased with 40 mmol稬-1 DTT and 1.5 mg loading amount. The 1089 protein spots were stained with Colloidal coomassie blue and detected by mass spectrometry.
- Kandelia candel,
- roots,
- Two-dimensional polyareylamide gel electrophoresis,
- Proteome

FullText(HTML)

References(2)

References

[1]	LIU H, LIU Y Z, ZHEN S Q, et al. Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion[J]. Planta, 2010, 231: 847-860.
[2]	CARPENTIER S C, WITTEM E, LAUKENS K, et al. Preparation of protein extracts from recalcitrant plant tissues: An evaluation of different methods for two-dimensional gel electrophoresis analysis[J].Proteomics,2005,5:2497-2507.