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Volume 27 Issue 8
Nov.  2012
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Article Contents
LI Hua-wei, LUO Wen-bin, JI Rong-chang, LIU Zhong-hua, QIU Si-xin, TANG Hao. Analysis of Genetic and Construction of Fingerprinting with SSR Markers for New Potato Varieties in Fujian Province[J]. Fujian Journal of Agricultural Sciences, 2012, 27(8): 810-814.
Citation: LI Hua-wei, LUO Wen-bin, JI Rong-chang, LIU Zhong-hua, QIU Si-xin, TANG Hao. Analysis of Genetic and Construction of Fingerprinting with SSR Markers for New Potato Varieties in Fujian Province[J]. Fujian Journal of Agricultural Sciences, 2012, 27(8): 810-814.

Analysis of Genetic and Construction of Fingerprinting with SSR Markers for New Potato Varieties in Fujian Province

  • Received Date: 2012-06-27
  • Rev Recd Date: 2012-07-23
  • Publish Date: 2012-08-30
  • For the purpose of new varieties(lines) identification at the molecular level, 11 pairs of SSR(Simple Sequence Repeats) primer markers were employed to analysis of genetic and construction of fingerprinting for 9 new potato varieties and its parents in Fujian.. The results showed that, 89 alleles loci were amplified, including 84 polymorphic alleles, and the ratio of polymorphism reached 94.38%. The numbers of alleles site amplified with different primers was 5-15, and the average value was 8.09. The fingerprinting of 13 potato varieties was constructed by 6 pairs of primers,STI027, STI052, STI033, STI047, STM3023, and STM0030. All the potato varieties were univocally identified using SSR primer STI027, and ten potato varieties were univocally identified using SSR primer STM0030. UPGMA cluster analysis of genetic similarity showed that all the materials were clustered into four groups at the genetic similarity of 0.628. The cluster analysis result was accordance with the genetic relationships.
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    段艳凤,刘杰,卞春松,等.中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457.
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    刘福翠,谭学林,郭华春.云南省马铃薯品种资源的RAPD分析[J].西南农业学报,2004,33(6):1349-1352.
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    李凤云,盛万民,刘昭军,等.马铃薯品种遗传多样性的AFLP分析[J].中国农学通报,2007,23(8):58-61.
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