• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 27 Issue 6
Aug.  2012
Turn off MathJax
Article Contents
WU Xue-min, ZHUANG Pei-de, MAO Ning, LIN Tian-long, GONG Hui, YANG Jin-xian. Biological Functions of Mah-TrxA Fusion Protein from Aeromonas hydrophila[J]. Fujian Journal of Agricultural Sciences, 2012, 27(6): 583-588.
Citation: WU Xue-min, ZHUANG Pei-de, MAO Ning, LIN Tian-long, GONG Hui, YANG Jin-xian. Biological Functions of Mah-TrxA Fusion Protein from Aeromonas hydrophila[J]. Fujian Journal of Agricultural Sciences, 2012, 27(6): 583-588.

Biological Functions of Mah-TrxA Fusion Protein from Aeromonas hydrophila

  • Received Date: 2012-05-08
  • Rev Recd Date: 2012-06-01
  • Publish Date: 2012-06-30
  • This experimentation was designed to help understand the antigenic and immunogenic rules of Aeromonas hydrophila (Ah). Indirect enzyme-linked immunosorbent assay showed the ratios of the antibody titers of the rabbit anti-ZN1-outer membrane protein (OMP) serum to the expression product, Mah-TrxA, and the recombinant protein of thioredoxin and a major adhesion agent and ZN1-OMP to be 1∶3 200 and 1∶12 800, respectively; and the ratios of the antibody titers of the rabbit anti- Mah-TrxA serum to Mah-TrxA and ZN1-OMP to be 1∶12 800 and 1∶1 600, respectively.Carp epithelial tumor cells (EPC) were used to study the adhesion effect of different sources and serotypes of Ah to EPC, as well as the biological functions played by Mah-TrxA.The adhesion test indicated that Ah were separated from the fish, the serotypes were O∶Ah10501, O∶9, and O∶CQ-1; the adhesion rate of Ah to EPC was greater than 97%; ATCC7966 was separated from milk, the serotype was O∶1; and, the adhesion rate of ATCC7966 to EPC was 78.5%.The results indicated that Mah-TrxA and the rabbit anti-Mah-TrxA serum could significantly inhibit or block the bacterial adhesion to EPC.It suggested that the fusion protein expressed through genetic engineering techniques in Ah had similar antigenicity and immunogenicity as the wild strain, ZN1.
  • loading
  • [1]
    陆承平. 致病性嗜水气单胞菌及其所致鱼病[J]. 水产学报, 1992, 16(3):282-288.
    [2]
    ALICIA AGUILAR, SUSANA MERINO, XAVIER RUBIRES,et al.Influence of Osmolarity on lipoplysac-charides and Virulence of Aeromonas hydrophila Serotype O∶34 Strains Grown at 37℃[J]. Infection and Immunity, 1997, 65 (4):1245-1250.
    [3]
    庄培德, 杨金先, 吴学敏, 等. 嗜水气单胞菌主要粘附素基因克隆表达及产物粘附功能分析[J]. 中国人兽共患病学报, 2011, 27(2):112-116.
    [4]
    顾天钊, 陆承平, 陈怀青. 鲍氏不动杆菌MF1株的粘附特性[J]. 南京农业大学学报, 1999, 22(3): 65-68.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (8236) PDF downloads(434) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return