Growth Factors, bFGF and IFG-1, in Pectoral Fin Passage Cells of Anguilla anguilla

To establish a model for cell isolation and identification, as well as to gain an in-depth understanding of the viral diseases in Anguilla anguilla, the roles played by the growth factors, bFGF and IFG-1, on the proliferation of the eel's pectoral fin cells were studied.Under in vitro conditions, the cells were collected, counted and growth curves constructed.On the L-15 medium containing 10% FBS, bFGF showed a significant evidence in promoting the growth of the pectoral fin cells.The cellular growth peaked at bFGF concentration of 10 μg·L-1.On the other hand, IGF-1 in the concentrations from 5 μg·L-1 to 50 μg·L-1 did not show any apparent effect on the growth.Therefore, a culture medium formulation for effective proliferation of the A.anguilla pectoral fin cells was obtained.This would, in turn, aid future studies on the pathogen's isolation, identification and vaccine preparation.