Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
XU Hui-bin, WEI Yi-dong, LIAN Ling, HE Wei, ZHANG Jian-fu, XIE Hua-an. Development of High Frequency Transformation of Indica Rice Mediated by Agrobacterium[J]. Fujian Journal of Agricultural Sciences, 2012, 27(5): 475-480.
Citation:
XU Hui-bin, WEI Yi-dong, LIAN Ling, HE Wei, ZHANG Jian-fu, XIE Hua-an. Development of High Frequency Transformation of Indica Rice Mediated by Agrobacterium[J]. Fujian Journal of Agricultural Sciences, 2012, 27(5): 475-480.
XU Hui-bin, WEI Yi-dong, LIAN Ling, HE Wei, ZHANG Jian-fu, XIE Hua-an. Development of High Frequency Transformation of Indica Rice Mediated by Agrobacterium[J]. Fujian Journal of Agricultural Sciences, 2012, 27(5): 475-480.
Citation:
XU Hui-bin, WEI Yi-dong, LIAN Ling, HE Wei, ZHANG Jian-fu, XIE Hua-an. Development of High Frequency Transformation of Indica Rice Mediated by Agrobacterium[J]. Fujian Journal of Agricultural Sciences, 2012, 27(5): 475-480.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China;
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China;
3.
Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, P.R.China, Fuzhou, Fujian 350003, China;
4.
National Rice Engineering Laboratory of China, Fuzhou, Fujian 350003, China;
5.
South China Bases of National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian 350003, China
The major factors were analyzed and compared affecting the transformation frequency of indica rice mediated by Agrobacterium including medium, culture condition, subculture frequency, bacterial concentration and infection time and so on in the paper. At last a high transformation frequency of indica rice was set up. The results indicated that NMB medium for callus inducing and NB medium for callus subculturing were the best medium combination for tissue culture of indica rice. In the meanwhile, bacterial concentration, infection time and concentration ratio of KT and NAA in differential medium at the best level were modulated. The transformed calli were pre-differentiation. So the differentiation rate was improved significantly. The albino seedlings were reduced. Finally the transformation rate of indica rice was improved to 8%.
DownLoad: