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Volume 27 Issue 4
Jun.  2012
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Article Contents
WU Jing-jing, HONG Zhi-hong, ZHANG Ka, CHEN Hong-wen. Cloning and Bioinformatic Analysis of 6-Phogluconate Dehydrogenase Gene from Aspergillus oryzae[J]. Fujian Journal of Agricultural Sciences, 2012, 27(4): 394-399.
Citation: WU Jing-jing, HONG Zhi-hong, ZHANG Ka, CHEN Hong-wen. Cloning and Bioinformatic Analysis of 6-Phogluconate Dehydrogenase Gene from Aspergillus oryzae[J]. Fujian Journal of Agricultural Sciences, 2012, 27(4): 394-399.

Cloning and Bioinformatic Analysis of 6-Phogluconate Dehydrogenase Gene from Aspergillus oryzae

  • Received Date: 2012-04-05
  • Rev Recd Date: 2012-04-16
  • Publish Date: 2012-04-30
  • 6-Phogluconate dehydrogenase (6PGDH, EC 1.1.1.44)is one of the key enzymes in the pentose phosphate pathway(PPP). In this study, the 6-phogluconate dehydrogenase gene (gnd) of Aspergillus oryzae CICC2012 was cloned by means of PCR. Subsequently, the bioinformatic methodology was applied to determine the amino acid sequence homology, phylogenetic trees, physical-chemical properties and protein structure of the gene. The results revealed the gene’s length to be 1 723 bp, which encompassed an open reading frame with 1 551 bp encoding 516 amino acids. The 6PGDH encoded by gnd showed a 99% homology with the 6PGDH gene of Aspergillus flavus, which had 11 serine phosphorylation sites, 2 threonine phosphorylation sites and 6 tyrosine phosphorylation sites. The 6PGDH had a molecular weight of 57.3 kD and an isoelectric point of 5.63. The secondary structure of the gnd protein was 44.57% alpha helix, 12.79% beta sheet and 42.64% random coil. The 11-195 amino acid residues appeared to be the NADP+ binding sites.
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    黄骥,王建飞,张红生.植物戊糖磷酸途径及两个关键酶的研究进展[J].植物学通报, 2004, 21(2): 139-145.
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    CHRISTOPHE D,ELIANA R,STEFANIA H,et al.Synthesis and biological evaluation of substrate-based inhibitors of 6-phosphogluconate dehydrogenase as potential drugs against African Trypanosomiasis[J]. Bioorganic & Medicinal Chemistry, 2003, 11: 3205-3214.
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    JUNKO O, RITSUKO K, SATOSHI M, et al. A novel gnd mutation leading to increased L L-lysine production in Corynebacterium glutamicum[J]. FEMS Microbiology Letters, 2005, 242: 265-274.
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    袁洪水,李术娜,张爱莲,等.石英砂研磨法快速提取顶头孢霉染色体DNA[J].河北农业大学学报, 2007, 30(5): 8-11.
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