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Volume 27 Issue 4
Jun.  2012
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Article Contents
ZHANG Li-jie, LI Tao, WEI Xiu-qing, ZHANG Xi-juan, CHEN Zhi-feng, XU Jia-hui. ISSR-PCR Optimization for Waxapple (Syzygium samarangense)[J]. Fujian Journal of Agricultural Sciences, 2012, 27(4): 343-349.
Citation: ZHANG Li-jie, LI Tao, WEI Xiu-qing, ZHANG Xi-juan, CHEN Zhi-feng, XU Jia-hui. ISSR-PCR Optimization for Waxapple (Syzygium samarangense)[J]. Fujian Journal of Agricultural Sciences, 2012, 27(4): 343-349.

ISSR-PCR Optimization for Waxapple (Syzygium samarangense)

  • Received Date: 2012-01-04
  • Rev Recd Date: 2012-03-27
  • Publish Date: 2012-04-30
  • An optimization experiment was conducted on the waxapple cultivars, Nong Ke NO 1. Genomic DNA was extracted from the young leaves using the modified CTAB method. Orthogonal design was applied to optimize ISSR-PCR amplification of the waxapple with 5 factors, including Mg2+,dNTPs,primer,Taq DNA polymerase and template DNA, at 4 levels. An optimal reaction system (25 μL) was established, i. e., 2.0 μL 10×buffer (Mg2+ free),3.0 mmol·L-1 Mg2+,0.2 mmol·L-1 dNTPs,0.4 μmol·L-1 primer,1.5 U Taq DNA polymerase and 30 ng template DNA. Waxapple cultivars, Nong Ke NO 1 and Nong Ke NO 2, were used to challenge the optimized reaction system and select primers. The results indicated that the system was stable and 12 primers were selected.
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  • [1]
    何桥,梁国鲁,谢江辉. 莲雾种质资源遗传多样性的ISSR分析[J]. 园艺学报, 2006, 33(2): 392-394.
    [2]
    LUISA M C, LUIS G, CRISTINA O. ISSR analysis of cultivars of pear and suitability of molecular markers for clone discrimination[J]. J Amer Soc Hort Sci, 2001, 126(5):517-522.
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