DNA Fingerprinting and Genetic Diversity of Cultivated Strains of Agaricus blazei Murill
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Graphical Abstract
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Abstract
Sixteen different strains of Agaricus blazei Murill were analyzed using ISSR, RAPD and SRAP. PCR amplification using 8 RAPD primers, 4 ISSR primers and 2 SRAP primer pairs separated the strains into three groups. One of them consisted of A0011+1 and A0013, another A0009, and the third group the remainders. The result showed that SRAP provided rich genetic information, and the RAPD primers had the largest number of polymorphic bands among all markers.
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