• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 27 Issue 2
Mar.  2012
Turn off MathJax
Article Contents
ZHANG Xiao-pei, FANG Qin-mei, GONG Hu, LIN Tian-long. Prokaryotic Expression and Immunological Analysis of the Flagella Gene of Edwardsiella tarda[J]. Fujian Journal of Agricultural Sciences, 2012, 27(2): 113-118.
Citation: ZHANG Xiao-pei, FANG Qin-mei, GONG Hu, LIN Tian-long. Prokaryotic Expression and Immunological Analysis of the Flagella Gene of Edwardsiella tarda[J]. Fujian Journal of Agricultural Sciences, 2012, 27(2): 113-118.

Prokaryotic Expression and Immunological Analysis of the Flagella Gene of Edwardsiella tarda

  • Received Date: 2011-09-02
  • Rev Recd Date: 2011-12-19
  • Publish Date: 2012-02-29
  • Edwardsiella tarda strain ETY was isolated from Japanese eel. With two pairs of specific primers, the flagella gene(ETF) was amplified from the strain ETY via nest-PCR. After sequencing analysis, the nucleotide data had been further analyzed by DNAman and ClutalW software. The analysis results showed that ETF had a longest open reading frame (ORF) of 1 257 bp, which was predicted to encode a 419-aa protein with the molecular weight of 43.951 kD. Considering ELISA (enzyme-linked immunosorbent assay) analysis and Western-blotting analysis, it was proved that the expressed gene products shared similar antigenicity and immunocompetence with the natural flagella. The protective rate in the Japanese eel immunized with the ETF-TrxA fusion protein and ISA adjuvant is 100%. It is the first time that the ETF gene was efficient expressed in E.coli., and it also primarily studied the ETF function of Edwardsiella tarda in invasion and inducing immunoreaction. The results enable the development and formulation of an appropriate and effective subunit vaccine(s) against Japanese eel edwardsiellosis.
  • loading
  • [1]
    [2]
    [3]
    林清华.免疫学实验[M].武汉:武汉大学出版社出版.1999:33-39.
    [4]
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2498) PDF downloads(492) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return