CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO AVIAN LEUKOSIS VIRUSES

Cheng Youquan; Lucy F. lee; Robert F. Silva; Fugene J. Smith; Lyman B. Crittenden

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Article Navigation > Fujian Journal of Agricultural Sciences > 1986 > 1(1): 1-10

Cheng Youquan, Lucy F. lee, Robert F. Silva, Fugene J. Smith, Lyman B. Crittenden. CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO AVIAN LEUKOSIS VIRUSES[J]. Fujian Journal of Agricultural Sciences, 1986, 1(1): 1-10.

Citation:

Cheng Youquan, Lucy F. lee, Robert F. Silva, Fugene J. Smith, Lyman B. Crittenden. CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO AVIAN LEUKOSIS VIRUSES[J]. Fujian Journal of Agricultural Sciences, 1986, 1(1): 1-10.

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CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO AVIAN LEUKOSIS VIRUSES

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences;
2.
U. S. Department of Agriculture, Agricultural Research Service, Regional Poultry Research Laboratory

Publish Date: 1986-09-15

Abstract

Abstract

Hybridoma cell lines secreting monoclonal antibody (MCA) to avian leukosis virus (ALV) structural proteins p27 and p19 have been established. MCA 6AL20 (IgG1 isotype) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV (CZ-RSV).representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 (subgroup E) in an indirect enzyme-linked immunosorbent assay (ELISA). MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus (PrC-RSV), subgroup C. Both MCAs im-munoprecipitated p19 from 35S-methionine-labeled RPL-40 or RAV-1,but not RAV-O infected chi-cken embryo fibroblasts (CEF). They can be used to differentiate exogenous from endogenous (RAV-O) infection either in an indirect antibody ELISA or by immunoprecipitation. A third MCA, 6AL42 (IgG2a isotype), reacted with exogenous viruses at an antibody titer up to 1,000-fold higher than with endogenous subgroup E, RAV-O virus in indirect ELISAs. MCA 6AL42 immunoprecipitated p27 and the group-specific antigen precursor protein, pr76, from cells infected with RPL-40, RAV-1 or RAV-O. A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.

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References(8)

References

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