THE PROMOTIVE EFFECTS OF ABSCISIC ACID ON RAPID CLONAL IN VITRO PROPAGATION OF Freesia refracta

The promotive effects of abscisic acid on bud formation of common freesia callus were studied. The results are summarized as follows:1.The floral envelope was cultured on MS medium supplemented with BA 1 mg/1 and NAA 0,1 mg/1, The frequency of culli induction and bud formation were a little. But the floral axis of the common freesia cultured on MS medium added with IBA, IBA greatly stimulated root formation in the concentration of 0.5-2 mg/1.2.When MS medium added with BA 0.1 mg/1 and 2,4-D 0.1 mg/1, the activated carbon played an important role, as to promote the culli induction and bud formation. The optimum concentration of the activated carbon was 0.8%.3.Abscisis acid is obviously beneficial to promote the bud strong in the concentration of 5 mg/1.