Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
Cheng Youuuquan, Zhuang Xiangsheng, Wu Ping, Lin Tianlong, Li Yiying, Huang Nin. Microball Double Antibody Sandwich Elisa for the Detection of Newcastle Disease Virus Antigen in Chickens[J]. Fujian Journal of Agricultural Sciences, 1991, 6(1): 27-34.
Citation:
Cheng Youuuquan, Zhuang Xiangsheng, Wu Ping, Lin Tianlong, Li Yiying, Huang Nin. Microball Double Antibody Sandwich Elisa for the Detection of Newcastle Disease Virus Antigen in Chickens[J]. Fujian Journal of Agricultural Sciences, 1991, 6(1): 27-34.
Cheng Youuuquan, Zhuang Xiangsheng, Wu Ping, Lin Tianlong, Li Yiying, Huang Nin. Microball Double Antibody Sandwich Elisa for the Detection of Newcastle Disease Virus Antigen in Chickens[J]. Fujian Journal of Agricultural Sciences, 1991, 6(1): 27-34.
Citation:
Cheng Youuuquan, Zhuang Xiangsheng, Wu Ping, Lin Tianlong, Li Yiying, Huang Nin. Microball Double Antibody Sandwich Elisa for the Detection of Newcastle Disease Virus Antigen in Chickens[J]. Fujian Journal of Agricultural Sciences, 1991, 6(1): 27-34.
A mieroball double antibody sandwich ELISA (MDS-ELISA) was developed to detect Newcastle disease virus (NDV) antigen in the tissues of chickens infected with NDV. In the assay, a monoclonal antibody (specific for NDV.IgM isotype), and its conjugate of horse radish peroxidase (FN-HRP) were served as the capture antibody and the indicator antibody, respectively. When FN was at 400g/ml, the detectable antigen amount was as low as 3g NDV protein per millilitre.The results of detection of NDV antigen from NDV-infected chickens by the MDS-ELISA and the direct immunof luorescent-antibody test (DFA) showed that the sensitivity of the MDS-ELISA was higher than of the DFA. All samples that were positive inthe DFA were also positive in the MDS-ELlSA. However, the MDS-ELISA indicated that a small number of samples were NDV antigen positive which were negative by the DFA. The concordance rate between tese two methods was 92%.The MDS-ELISA was evaluated on detection of the samples from chickens inoculated with various NDV strains. The experiment demonstrated that: 1) the positive rates in the various organs from the chickens infected with F48 strain of NDV were 100% in a mixture of lung, kidney and ileocecal tonsil, and in kidney. 90% in lung, 80% in ileo-cecal tonsil, liver and spleen, respectively; 2) only some positive samples were found in Mukteswar-inoculated chickens in 6 days post-injection; 3) no positive samples had been found in Bl strain infected chickens.The MDS-ELISA was proved to be a most sensitive, specific, rapid and convenient method. It would be most useful for diagnosis of ND in poultry farms.