Multiplication of <i>Anabaena Azollae</i> in Vitro

Tang Longfei; Liu Zhongzhu; I.Watanabe

Volume 6 Issue 2

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Tang Longfei, Liu Zhongzhu, I.Watanabe. Multiplication of Anabaena Azollae in Vitro[J]. Fujian Journal of Agricultural Sciences, 1991, 6(2): 25-32.

Citation:

Tang Longfei, Liu Zhongzhu, I.Watanabe. Multiplication of Anabaena Azollae in Vitro[J]. Fujian Journal of Agricultural Sciences, 1991, 6(2): 25-32.

Tang Longfei, Liu Zhongzhu, I.Watanabe. Multiplication of Anabaena Azollae in Vitro[J]. Fujian Journal of Agricultural Sciences, 1991, 6(2): 25-32.

Citation:

Tang Longfei, Liu Zhongzhu, I.Watanabe. Multiplication of Anabaena Azollae in Vitro[J]. Fujian Journal of Agricultural Sciences, 1991, 6(2): 25-32.

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Multiplication of Anabaena Azollae in Vitro

1.
National Azolla Research Center, Fujian Academy of Agricultural Sciences, Fuzhou, 350013;
2.
International Rice Research Institute, Manila 1099

Received Date: 1990-12-15
Publish Date: 1991-11-15

Abstract

Abstract

This experiment tried to isolate Andbaena azollae directly from Azolla leaf cavities after surface sterilization, then grew it on various culture media and conditions.More longer green-keeping periord of isolated Anabdena azollae was found in alien medium suplemented with organic N and C, micro-aerobic(1% O2, 99% N2)condition to compare with N-free medium and aerobic condition.The isolates underwent a limited multiplication in above growing condition but failed to continue the further multiplication.These cyanobacteria were antigenically identical to the endosybionts of Azolla.
- Anabaena azollae,
- Monoclonal antibody,
- artificial cultivation,
- Limited mutiplication

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References(2)

References

[9] Bai K. Z., S. L. Yu, W. L. Chen and S. Y. Yang. 1978. Cultivation of alga-free A. wlla, isolation of Anabaena azollae and preliminary attempt at their association. In Proc. Symposium on Plant 'Tissue Culture. Science Press. Beijing. pp 455-458

[11] Franche C., and G. Cohen-Bazite. 1985. The structural nif genes of four symbiotic Anabaena awllae show highly conserved physical arrangement. Plant Sci., 39: 125-131

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