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ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.
Citation:
ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.
ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.
Citation:
ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.
Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China
Conservative fragment of VP5 gene was amplified by RT-PCR and cloned into pMD18-T vector.After sequencing,the positive recombinant plasmid was acquired and used to establish standard and melt curves of the real-time fluorescent quantitative RT-PCR.The standard curve for the threshold cycle and viral genomic copy number ranging from 3.2910~3.29108 were linear.The sensitivity of detection was 33 copies.It was concluded that the real time RT-PCR SYBR GreenⅠ technology was highly specific and repeatable for IBDV diagnostics and quantification.
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