• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 26 Issue 2
Nov.  2011
Turn off MathJax
Article Contents
ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.
Citation: ZHU Chun-hua, LIN Su, CHENG Long-fei, CHEN Hong-mei, CHEN Zhen, LIU Bin-qiong, CAI Guo-zhang, LIN Yu, HUANG Yu. Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 175-179.

Detection of Infectious Bursal Disease Virus Using RT-PCR SYBR GreenⅠ Technology

  • Received Date: 2011-03-02
  • Rev Recd Date: 2011-03-15
  • Publish Date: 2011-04-15
  • Conservative fragment of VP5 gene was amplified by RT-PCR and cloned into pMD18-T vector.After sequencing,the positive recombinant plasmid was acquired and used to establish standard and melt curves of the real-time fluorescent quantitative RT-PCR.The standard curve for the threshold cycle and viral genomic copy number ranging from 3.2910~3.29108 were linear.The sensitivity of detection was 33 copies.It was concluded that the real time RT-PCR SYBR GreenⅠ technology was highly specific and repeatable for IBDV diagnostics and quantification.
  • loading
  • [1]
    [2]
    ROJS O Z,KRAPEZ U,SLAVEC B,et al.Molecular characterisation of infectious bursal disease viruses isolated in recent acute outbreaks in Slovenia[J].Acta Vet Hung,2008,56 (2):255-264.
    [3]
    何秀苗,韦平,官丁明,等.2000~2007年广西鸡传染性法氏囊病病毒的分子流行病学[J].病毒学报,2009(6):437-444.
    [4]
    [5]
    WANG M Y,HUH L,SUEN S Y,et al.Development of an enzyme-linked immunosorbent assay for detecting infectious bursal disease virus (IBDV) infection based on the VP3 structural protein[J].Vet Microbiol,2008,131 (3-4):229-236.
    [6]
    王永强,祁小乐,高宏雷,等.鸡传染性法氏囊病病毒实时荧光定量RT-PCR检测方法的建立[J].中国预防兽医学报,2009,31(1):65-68.
    [7]
    [8]
    SHI L,LI H,MA G,et al.Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts[J].Virus Genes,2009,39 (1):46-52.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2862) PDF downloads(846) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return