• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 26 Issue 2
Nov.  2011
Turn off MathJax
Article Contents
HU Chang-quan, LIU Hua qing, LI Su yi, PAN Da ren, WANG Feng. Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 143-147.
Citation: HU Chang-quan, LIU Hua qing, LI Su yi, PAN Da ren, WANG Feng. Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 143-147.

Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging

  • Received Date: 2011-01-12
  • Rev Recd Date: 2011-02-17
  • Publish Date: 2011-04-15
  • Binary vector pCAMBIA1300GUSA-Hyg containing β-glucuronidase(GUS) without the promoter was introduced into Oryza sativa L.Minghui 86 by agrobacterium mediated transformation.The χ2 tests on the hygromycin resistant ratio revealed that 28 of the 39 rice promoter trap lines contained a loci T-DNA tagging.GUS histochemical assay at different stages in different organs,such as root,stem,leave,anther,hull,embryo and emdospern,of 2653 rice promoter trap lines was performed.The results showed that 83 rice promoter lines were GUS positive.Of all tested,24 lines were expressed in endosperm(i.e.,0.91%) and 19 lines expressed in anther(i.e.,0.72%).Information collected on the rice promoter trap lines could be useful in identifying new rice genes.
  • loading
  • [1]
    JEON JS,LEE S,JUNG KH,et al.T-DNA insertional mutagenesis for functional genomics in rice[J].Plant J,2000,22 (6):561-70.
    [2]
    SPRINGER PS.Gene traps:tool for plant development and genomics[J].Plant Cell,2000,12:1007-1020.
    [3]
    JEFFERSON R A.Assaying chimeric genes in plants:the GUS gene fusion system[J].Plant Molecular Biology Reporter,1987,5:387.
    [4]
    ZHANG J,LI C,WU C,et al.RMD:a rice mutant database for functional analysis of the rice genome[J].Nucleic Acids Res,2006,1 (34):745-748.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2588) PDF downloads(685) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return